Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Effect of pioglitazone on antioxidant capacity and oxidative damage after spinal cord injury in rat

(2018) Effect of pioglitazone on antioxidant capacity and oxidative damage after spinal cord injury in rat. Journal of Babol University of Medical Sciences. pp. 16-22. ISSN 15614107 (ISSN)

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Abstract

BACKGROUND AND OBJECTIVE: Reduction of the antioxidant capacity and oxidative damage has a crucial role in development of damage after spinal cord injury. Since pioglitazone (PPAR-gamma agonist) have a powerful antioxidant property, the present study aimed to evaluate the effect of pioglitazone on antioxidant capacity and oxidative damage in the injured areas of spinal cord in rat. METHODS: In the present experimental study eighteen male Wistar rats divided into three groups as follow (n=6); sham, control injured and pioglitazone-treated injured group. Spinal cord injury was performed according to the Ping-Weight Drop (contusion) model in rat. The animals received pioglitazone (3 mg/kg) intraperitoneally at times of 15 min after injury and then each 12 hours until a week. At the end, malondialdehyde level, activity of catalase and superoxide dismutase (SOD) enzymes and also histopathological alterations of spinal cord were assessed. FINDINGS: Induction of spinal cord injury in control injured animals significantly increased the malondialdehyde levels (56) and decreased the activity of catalase (48) and SOD (65) enzymes compared to sham group (P=0.004, P=0.001 and P=0.008, respectively). Pioglitazone in treated injured group significantly decreased the malondialdehyde levels (38) and increased the activity of catalase (34) enzyme compared to control injured group (P=0.038 and P=0.014, respectively). Also, pioglitazone prevented the histopathological changes of injured areas in spinal cord. CONCLUSION: The findings of present study indicate that treatment with pioglitazone through potentiation of the antioxidant defense capacity of injured spinal cord decreases oxidative damage and also histopathological changes of spinal cord. © 2018, Babol University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: Antioxidant capacity Malondialdehyde Oxidative damage Pioglitazone Spinal cord injury catalase free radical malonaldehyde placebo superoxide dismutase animal experiment animal model animal tissue antioxidant activity Article controlled study enzyme activity experimental spinal cord injury histopathology laminectomy lipid peroxidation male nerve lesion nonhuman oxidative stress rat
Divisions:
Page Range: pp. 16-22
Journal or Publication Title: Journal of Babol University of Medical Sciences
Journal Index: Scopus
Volume: 20
Number: 5
ISSN: 15614107 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/675

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