Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Production and Evaluation of Anti-Salmonella Typhimuruim Immunoglobulin Y Isolated from Serum and Eggs Laid

(2009) Production and Evaluation of Anti-Salmonella Typhimuruim Immunoglobulin Y Isolated from Serum and Eggs Laid. Yakhteh. pp. 260-265. ISSN 1561-4921

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Abstract

Objective: The purpose of the study was to evaluate the production of antibodies in serum as well as egg yolk raised against S. typhimurium and the cross-reactivity of this antibody with other enteric bacteria. Materials and Methods: White egg-laying hens were immunized with S. typhimurium, heat-killed whole cell, in Freund's adjuvant. Immunization was done with 10(7) conlony forming unit (CFU) of bacteria per hen which was injected into the breast muscle of lay. Specific antibodies were detected by enzyme-linked immunosorbent assay in the serum and in eggs. The serum and eggs of two adults white Leghorn hens were not immunized with S. typhimurium used as a control. Results: Chicken egg yolk antibodies (IgY) were raised against S. typhimuruim in the serum as well as in eggs. The production of IgY in serum was higher than IgY produced in egg yolk. Anti-S. typhimurium IgY cross-reacted 63, 25 and 14 with S. typhimurium, Shigella dysenteriae and E. coli respectively. Conclusion: The findings indicate that eggs from hens immunized with S. typhimuruim have not specific antibodies for the detection of S. typhimuruim, but they may have the potential of being a useful source of passive immunity against this pathogen.

Item Type: Article
Keywords: Immunoglobulin Y (IgY) Salmonella ELISA Egg yolk antibodies passive-immunization streptococcus-mutans dental-caries enteritidis igy chickens protection vaccine trends Research & Experimental Medicine
Divisions:
Page Range: pp. 260-265
Journal or Publication Title: Yakhteh
Journal Index: ISI
Volume: 10
Number: 4
ISSN: 1561-4921
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6818

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