Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Ganoderma Lucidum Induces the Expression of CD40/CD86 on Peripheral Blood Monocytes

(2009) Ganoderma Lucidum Induces the Expression of CD40/CD86 on Peripheral Blood Monocytes. Iranian Journal of Immunology. pp. 87-91. ISSN 1735-1383

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Abstract

Background: The major immuno-modulating effects of Ganoderma lucidum include mitogenicity and activation of immune effector cells such as T cells, macrophages and natural killer cells resulting in the production of cytokines. Objective: The purpose of this study was to evaluate the expression of CD40 and CD80 by G. lucidum-treated human peripheral blood mononuclear cells. Methods: Monocytes were isolated and incubated at 37 degrees C and 5 CO(2) for 24 h and 48 h in the presence or absence of different concentrations of G. lucidum. Cells were then incubated with labelled monoclonal antibodies against CD14, CD40 and B7-1(CD80) molecules utilizing standard protocols, and analyzed by flow cytometry. Results: The results showed that incubation of monocytes with G. lucidum led to marked enhancement of CD40 and B7-1 expression in a dose- and time-dependent manner (p<0.001). G. lucidum was more effective in enhancing the expression of CD80 and CD40 molecules of cells obtained from females than male donors (p<0.001). Conclusion: G. lucidum enhanced the expression of CD40 and CD80 molecules on peripheral blood monocytic cells derived from both sexes in a dose-dependent manner, with a preferential higher effect on cells obtained from female donors.

Item Type: Article
Keywords: Ganoderma lucidum Monocytes CD14 CD40 CD80 miao-san supplementation prostate-cancer cells human dendritic cells nf-kappa-b rheumatoid-arthritis polysaccharide androgen receptor activation macrophage Immunology
Divisions:
Page Range: pp. 87-91
Journal or Publication Title: Iranian Journal of Immunology
Journal Index: ISI
Volume: 6
Number: 2
ISSN: 1735-1383
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6897

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