Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Determination of the inhibition and recovery of the plasma, cerebral cortex and hippocampus acetylcholinesterase activity in male paraoxon-treated rats

(2010) Determination of the inhibition and recovery of the plasma, cerebral cortex and hippocampus acetylcholinesterase activity in male paraoxon-treated rats. Journal of Babol University of Medical Sciences. pp. 65-70. ISSN 15614107 (ISSN)

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Abstract

BACKGROUND AND OBJECTIVE: Organophosphorus (OP) compounds are highly toxic and are widely used as an insecticide in agriculture and domestic consumptions. Acetylcholinesterase (AChE) inhibition is the primary mechanism of acute in vivo toxicity of organophosphorus compounds. In the present study we evaluated inhibition and recovery of the plasma, cerebral cortex and hippocampus AChE activity and their correlations following systemic administration of three doses of paraoxon in three different time points in rat. METHODS: Eighty four male Wistar rats (200-270 g) were used in this study and divided into groups of seven. Animals were given a single intraperitoneal injection of corn oil (vehicle group) or one of the doses of paraoxon (0.1, 0.3, or 0.7 mg/kg) and AChE activity in the plasma, cerebral cortex, and hippocampus was measured at 30 min, 4 h, and 18 h after exposure using the modified method of Ellman. FINDINGS: Plasma and brain AChE activity was inhibited in a dose dependent manner by paraoxon. After 18 h, plasma AChE activity was recovered 32, and 42 percent (p<0.001) in animals exposed to 0.3, and 0.7 mg/kg paraoxon, respectively. 18 h after 0.7 mg/kg paraoxon, AChE activity was significantly recovered (p<0.001) in both brain areas (about 20). Plasma AChE activity correlated significantly with both cerebral cortex and hippocampus AChE activity in rats treated with paraoxon (0.3 and 0.7 mg/kg). CONCLUSION: In both brain areas, paraoxon (only 0.7 mg/kg) inhibited AChE activity to induce seizure activity after 30 min. Inhibition of the plasma AChE activity can use as a marker of exposure only in severe toxicities with OP compounds.

Item Type: Article
Keywords: Acetylcholinesterase Cerebral cortex Hippocampus Paraoxon Recovery corn oil analytic method animal experiment article brain cortex concentration response controlled study enzyme activity enzyme inhibition male nonhuman plasma rat seizure
Divisions:
Page Range: pp. 65-70
Journal or Publication Title: Journal of Babol University of Medical Sciences
Journal Index: Scopus
Volume: 12
Number: 1
ISSN: 15614107 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/775

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