(2009) Rapid detection of Borrelia burgdorferi strains by nested polymerase chain reaction. Pakistan Journal of Biological Sciences. pp. 463-466. ISSN 10288880 (ISSN)
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Abstract
This study was carried out to evaluate the nested PCR for specific detection of different strains of B. burgdorferi. Five strains of B. burgdorferi including ACA-1, B-31, 2B45, 3B45, 7B49 obtained from different countries were used in this study. The strains of B. hermsii, Escherichia, coli and T. pallidum were also included as control strains. Two pairs of nested PCR primers were used to amplify the gene encoding the Osp-A protein of B. burgdorfer under standard PCR condition. In a two stage procedure, nested PCR yielded a positive reaction for five tested strains of B. burgdorferi. None the strains including B. hermsii, E. coli and 7. pallidum showed positive reaction when used as control strains in PCR. In conclusion, nested PCR showed acceptable specificity for rapid detection of B. burgdorferi. © 2009 Asian Network for Scientific Information.
| Item Type: | Article |
|---|---|
| Keywords: | Borrelia burgdorferi Lyme borreliosis PCR Rapid detection animal article bacterial gene classification genetics human isolation and purification Lyme disease methodology polymerase chain reaction Animals Genes, Bacterial Humans Escherichia coli Treponema pallidum |
| Divisions: | |
| Page Range: | pp. 463-466 |
| Journal or Publication Title: | Pakistan Journal of Biological Sciences |
| Journal Index: | Scopus |
| Volume: | 12 |
| Number: | 5 |
| Identification Number: | https://doi.org/10.3923/pjbs.2009.463.466 |
| ISSN: | 10288880 (ISSN) |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/816 |
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