(2020) Investigation of antibacterial effect and expression of alkaline protease gene in pseudomonas aeruginosa (Pao1) using copper oxide, copper complex and satureja khuzistanica: Rt-pcr method. International Journal of Pharmaceutical Research. pp. 1306-1321. ISSN 09752366 (ISSN)
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Abstract
Background and objective: In the genus Pseudomondaceae, exists Pseudomonas aeruginosa which is a gramnegative bacterium. It is an opportunistic human pathogen that frequently colonizes patients who are immunocompromised, such as those with cystic fibrosis, cancer or AIDS. Pseudomonas aeruginosa also has inherent resistance to several different chemotherapeutic agents and antibiotics. This study intended to evaluate the inhibition effect of copper oxide nanoparticle (CuO), copper nano complex (Cu complex) and the Satureja Khuzistanica essence on the gene of alkaline protease in P. aeruginosa (PAO1) by RT-PCR method. Method and material: Satureja Khuzistanica essence was synthesized by Clevenger apparatus. Nanoparticular size and morphology were characterized by the use of SEM. The antimicrobial activity of these substances was calculated from the process of disk diffusion and microbroth dilution based on CLSI norms. After determination of the Minimum Inhibitory Concentration (MIC) and Zone of Inhibition (ZOI), these data were analyzed using SPSS 21 software, through descriptive and inferential statistics at a 95 confidence level. In this study, ethical considerations were respected. Results: Zone of inhibition (ZOI) and Minimum inhibitory concentration (MIC) of Satureja Khuzistanica essence, CuO and Cu complex for PAO1 were found to be 19, 15 and 9 mm and 4, 12.5, and 25μg/ml respectively. Furthermore, the MIC synergism of Satureja Khuzistanica essence and CuO was 2 and 3.125μg/ml and Satureja Khuzistanica essence and Cu complex was 2 and 6.25μg/ml respectively. The ZOI synergism of Satureja Khuzistanica essence and CuO was 23mm and Satureja Khuzistanica essence and Cu complex were reported 20mm. Conclusion: According to the results obtained from the present study, a significant antibacterial activity was seen by Satureja Khuzistanica essence. By examining the synergism effect of CuO, Satureja khuzistanica essence, and Cu complex essence simultaneously, the further antimicrobial effect than was observed when each were used alone. © 2020, Advanced Scientific Research. All rights reserved.
| Item Type: | Article |
|---|---|
| Keywords: | Bacterium Chemotherapeutic agents Cu complex CuO alkaline proteinase amikacin ciprofloxacin complementary DNA copper complex copper oxide copper oxide nanoparticle gentamicin plant medicinal product polymyxin B Satureja Khuzistanica essence tobramycin unclassified drug antibacterial activity antibiotic sensitivity Article bacterium isolation comparative study controlled study crystal structure disk diffusion distillation DNA extraction drug potentiation enzyme linked immunosorbent assay Escherichia coli gene expression infrared spectroscopy minimum inhibitory concentration multiplex polymerase chain reaction nonhuman proton nuclear magnetic resonance Pseudomonas aeruginosa reverse transcription polymerase chain reaction savory scanning electron microscopy synergistic effect X ray powder diffraction zone of inhibition |
| Page Range: | pp. 1306-1321 |
| Journal or Publication Title: | International Journal of Pharmaceutical Research |
| Journal Index: | Scopus |
| Volume: | 12 |
| Number: | 4 |
| Identification Number: | https://doi.org/10.31838/ijpr/2020.12.04.067 |
| ISSN: | 09752366 (ISSN) |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/8337 |
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