Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Electrochemical Evaluation of Choline Oxidase on Modified Bamboo-Like CNTs Electrode: from Nano-Chemistry Towards Biotechnological Application

(2020) Electrochemical Evaluation of Choline Oxidase on Modified Bamboo-Like CNTs Electrode: from Nano-Chemistry Towards Biotechnological Application. Biointerface Research in Applied Chemistry. pp. 6252-6261. ISSN 2069-5837

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Abstract

Carbon nanotube (CNT) can be considered as one of the best materials for enzyme nanobiosensor. In this study, we investigated comprehensively electron transfer properties in three kinds of carbon nanotubes for finding the best supporting material for comprising of the electrochemistry and electrocatalysis of enzymes in nano-dimension. Choline oxidase was selected as a sample enzyme for evaluating the performance of the electrodes. The enzyme was absorbed on modified SWCNT, MWCNT and Bamboo-like CNTs electrode in ionic liquid. Electron transfer and electroanalytical responses were investigated by Metrohm Autolab. Functionalization was studied by Raman spectroscopy and FTIR and also, the morphological evaluation was conducted by SEM. Raman spectroscopy showed that Bamboo-like CNTs had better properties in loading enzyme and other electrochemical properties for electron transfer. FTIR spectroscopy showed that Bamboo-like CNTs had high covalent binding on the surface wall of nanotubes. High loading of the enzyme was detected in Bamboo-like CNTs in comparison to two other nano-tubes by SEM. The electrochemical and amperometric response showed that Bamboo-like CNTs had a better response in different scan rates, wide linear range and detection limit. ChOx/RTIL/Bamboo-like CNTs/GC electrodes showed the high amounts of enzyme loading, high enzyme-substrate affinity, low detection limit, best sensitivity and wider linear range.

Item Type: Article
Keywords: Bamboo-like CNTs Choline oxidase Electron transfer Ionic liquids CNTs Nanobiosensor carbon nanotubes ionic liquids hemoglobin Chemistry
Page Range: pp. 6252-6261
Journal or Publication Title: Biointerface Research in Applied Chemistry
Journal Index: ISI
Volume: 10
Number: 5
Identification Number: https://doi.org/10.33263/briac105.62086217
ISSN: 2069-5837
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/8542

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