Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

How preparation and preservation procedures affect the properties of amniotic membrane? How safe are the procedures?

(2020) How preparation and preservation procedures affect the properties of amniotic membrane? How safe are the procedures? Burns. pp. 1254-1271. ISSN 0305-4179

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Abstract

Human amniotic membrane (AM) has been widely used for tissue engineering and regenerative medicine applications. AM has many favorable characteristics such as high biocompatibility, antibacterial activity, anti-scarring property, immunomodulatory effects, anti-cancer behavior and contains several growth factors that make it an excellent natural candidate for wound healing. To date, various methods have been developed to prepare, preserve, cross-link and sterilize the AM. These methods remarkably affect the morphological, physico-chemical and biological properties of AM. Optimization of an effective and safe method for preparation and preservation of AM for a specific application is critical. In this review, the isolation, different methods of preparation, preservation, cross-linking and sterilization as well as their effects on properties of AM are well discussed. For each section, at least one effective and safe protocol is described in detail. (C) 2019 Elsevier Ltd and ISBI. All rights reserved.

Item Type: Article
Keywords: Human amniotic membrane Preparation Tissue engineering Regenerative medicine Preservation Decellularization limbal epithelial-cells ex-vivo expansion ocular surface cross-linking umbilical-cord antimicrobial activity biological-properties gamma-radiation gelatin films messenger-rna General & Internal Medicine Dermatology Surgery
Page Range: pp. 1254-1271
Journal or Publication Title: Burns
Journal Index: ISI
Volume: 46
Number: 6
Identification Number: https://doi.org/10.1016/j.burns.2019.07.005
ISSN: 0305-4179
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/8620

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