Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Prevention of nosocomial Acinetobacter baumannii infections with a conserved immunogenic fimbrial protein

(2020) Prevention of nosocomial Acinetobacter baumannii infections with a conserved immunogenic fimbrial protein. Apmis. pp. 476-483. ISSN 0903-4641

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Abstract

Acinetobacter baumannii, one of the most life-threatening nosocomial drug-resistant pathogens, imposes high morbidity and mortality rates, thus highlighting immunization-based treatments or prevention measures. The selection of appropriate antigens can elicit protective immunity. The gene encoding a fimbrial protein introduced via reverse vaccinology was cloned, expressed and evaluated for immunogenicity in a murine model. Mice immunized with the recombinant protein were challenged with A. baumannii ATCC 19606. Adherence to A549 cell line of specific anti-sera treated A. baumannii was also assessed. Passive immunity was evaluated in a murine pneumonia model. Indirect ELISA showed a high specific antibody titre. Adherence of A. baumannii to A549 cell line decreased by 40 after incubation with 1:250 dilution of specific anti-sera. All the actively immunized mice survived. Bacterial load in the spleen and liver of the immunized mice was 3-fold lower than those of the control. The number of bacteria in the lungs of passively immunized mice was about 6-fold lower than the control mice. The fimbrial protein could be considered as a promising protective immunogen against A. baumannii.

Item Type: Article
Keywords: Abaumannii bacterial vaccines fimbrial protein immunology molecular microbiology peptides vaccines design Immunology Microbiology Pathology
Page Range: pp. 476-483
Journal or Publication Title: Apmis
Journal Index: ISI
Volume: 128
Number: 7
Identification Number: https://doi.org/10.1111/apm.13061
ISSN: 0903-4641
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/8763

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