Abstract

Pseudomonas aeruginosa is a third hospital infection agent, and it is the second essential factor in wound infections. Despite many scientific advances in the treatment of burns, burn is one of the significant public health problems worldwide, particularly in developing countries. In this study, natural ointment ZOUSH was formulated with alcoholic extracts of Satureja khuzestaniea, Zataria multiflora and Mentha Mozaffariani Jamzad with Honey, and Polyurethane. A comparison between ZOUSH ointment and antibiotic was made by the disc diffusion method. PCR techniques carried out to confirm the genes exoS, lasA, and lasB, the housekeeping genes gyrA and fabD were used as a control. The burn wound was created by Ian Allen Holder method. The treatment was performed in 20 days, and the size of the burn was measured. Silver sulfadiazine ointment was considered as a positive control. The results indicated that 10, 13, 17 and 20 mu g concentration of ZOUSH ointment have 12, 14, 15, 18 mm inhibition zone. Also, ZOUSH ointment can decrease the size of the wound and has wound healing properties. Due to the effectiveness of ZOUSH ointment on the size of burn wound and its antibacterial properties on P. aeruginosa, this ointment is recommended for the treatment of burn wounds.