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Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Evaluation of Antibiotic Resistance and Biofilm Formation Ability Uropathogenic E. coli (UPEC) Isolated from Pregnant Women in Karaj

(2021) Evaluation of Antibiotic Resistance and Biofilm Formation Ability Uropathogenic E. coli (UPEC) Isolated from Pregnant Women in Karaj. Iranian Journal of Medical Microbiology. pp. 195-211. ISSN 17358612 (ISSN)

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Abstract

Background and Aim: Uropathogenic Escherichia coli (UPEC) are the most common cause of urinary tract infections. The binding of these bacteria to epithelial cells and the formation of biofilms cause these bacteria to be further colonized and difficult to remove in the urinary tract. This study aimed to determine the antibiotic resistance and to evaluate the biofilm formation power in Escherichia coli isolated from pregnant women in the city of Karaj, Iran. Materials and Methods: This descriptive-analytical study was performed on 64 isolates of UPEC. Identification of these bacteria was determined using biochemical tests and antibiotic resistance by Kirby – Bauer method and according to the recommendation of the Clinical and Laboratory Standards Institute (CLSI 2017) guideline. The ability to form biofilms, using the microtiter plate method and the presence of papC and sfa genes was examined by Duplex PCR. Results: Based on the results; the highest levels of resistance were related to cotrimoxazole (40.6), and ampicillin (31.3). The examination of biofilm formation by the phenotypic method also showed that of the isolates, 48.4 (strongly), 15.6 (moderately) and 21.8 (weakly) have biofilm formation power. Based on the obtained Duplex PCR results, 15 isolates had papC gene, 10 isolates had sfa gene and 9 isolates had both genes simultaneously. 100 of the isolates with both papC and sfa genes were able to produce biofilms. Conclusion: The results showed that most UPEC causing urinary tract infections can form biofilms. Also, the abundance of papC and sfa genes encoding pili in these strains can act as one of the binding factors of this bacterium. Copyright © 2021, This is an original open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License which permits copy and redistribution of the material just in noncommercial usages with proper citation.

Item Type: Article
Keywords: Antibiotic resistance Biofilm formation Duplex PCR Uropathogenic Escherichia coli (UPEC)
Page Range: pp. 195-211
Journal or Publication Title: Iranian Journal of Medical Microbiology
Journal Index: Scopus
Volume: 15
Number: 2
Identification Number: https://doi.org/10.30699/ijmm.15.2.195
ISSN: 17358612 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9249

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