Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Incorporation of graphene oxide and calcium phosphate in the PCL/PHBV core-shell nanofibers as bone tissue scaffold

(2021) Incorporation of graphene oxide and calcium phosphate in the PCL/PHBV core-shell nanofibers as bone tissue scaffold. Journal of Applied Polymer Science. ISSN 00218995 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Bone tissue scaffolds should have both desired mechanical stability and cell activities including biocompatibility, cell differentiation, and maturation. Also, suitable mineralization is another key factor for these materials. Hence, in current work, in order to achieve a scaffold with desired mechanical and bioactivity properties, core-shell nanofibers based on the polycaprolactone and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with different concentration of graphene oxide (GO) (0.5, 1, and 1.5 wt) and calcium phosphate (CP) (1 and 3 wt) were prepared to utilize as bone scaffold. Microstructure of nanofibers observed by field emission scanning electron microscope (FE-SEM) and results exhibited that the most of nanofibers had 270–500 nm diameter. Attenuated total reflectance Fourier transform infrared spectroscopy and energy dispersive X-ray evaluations verified appearance of GO and CP into the electrospun scaffolds (ES). Transmission electron microscopy analysis endorsed core-shell structure of nanofibers. X-ray diffraction study moreover determination of semicrystalline structure, verified presence of GO and CaPO4 into the nanofibers. Water contact angle demonstrates that, ES2 and ES3 situated in suitable domain of hydrophilicity. Tensile analysis determined that, ES2, ES3, and ES4 had the highest mechanical properties for use as bone scaffold. Cell viability assessment confirmed biocompatibility of scaffold during 7 days. Alkaline phosphatase and alizarin red staining exhibited maturating and differentiating of osteocytes after 21 days seeding on the scaffolds. © 2020 Wiley Periodicals LLC

Item Type: Article
Keywords: bone scaffold core-shell tissue engineering Biocompatibility Biomechanics Bone Calcium phosphate Contact angle Fourier transform infrared spectroscopy Graphene High resolution transmission electron microscopy Mechanical stability Nanofibers Phosphatases Scanning electron microscopy Shells (structures) Tissue Attenuated total reflectance Fourier transform infrared spectroscopy Bone tissue scaffolds Core-shell nanofibers Energy dispersive x-ray Field emission scanning electron microscopes Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Semicrystalline structures X-ray diffraction studies Scaffolds (biology)
Journal or Publication Title: Journal of Applied Polymer Science
Journal Index: Scopus
Volume: 138
Number: 6
Identification Number: https://doi.org/10.1002/app.49797
ISSN: 00218995 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9257

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