Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Current and emerging technologies for the diagnosis of sars-cov-2

(2021) Current and emerging technologies for the diagnosis of sars-cov-2. Open Microbiology Journal. pp. 77-86. ISSN 18742858 (ISSN)

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Currently, there are numerous under development or developed assays with various sensitivities and specificities for diagnosis of the Coronavirus Disease 2019 (COVID-19) caused by the SARS-CoV-2 virus. The World Health Organization (WHO) has approved several detection protocols based on real-time reverse transcription PCR (RT-qPCR) and the reliability of tests to detect the N, S, or RdRp/Hel genes of the SARS-Cov-2 virus has also investigated. Among these targets, COVID-19-RdRp/Hel targets represented the highest sensitivity. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has also been developed to rapidly and efficiently amplify RNA under isothermal conditions. Other isothermal amplification approaches such as nucleic acid sequence-based amplification (NASBA), recombinase polymerase amplification (RPA), and rolling circle amplification (RCA) have also been reported for detecting coronaviruses but like LAMP assay. Different serological tests, including neutralization tests, immunofluorescent (IFA), enzyme-linked immunosorbent (ELISA), and western blotting assays, are available. Point-of-care tests (POCT) are emerging to detect the virus genome, IgG, or IgM antibodies against SARS-CoV-2. The advent of more sensitive, cheaper, and easier-to-perform diagnostic tests seems to be a fundamental prerequisite to improve the diagnosis of COVID-19 infection. Herein, we reviewed several commercially available diagnostic methods used in many clinical laboratories to detect COVID-19. © 2021 Afshar et al.

Item Type: Article
Keywords: Bronchoalveolar Lavage COVID-19 CRISPRcas9 Detection methods LAMP assay Real-time reverse transcription PCR C reactive protein gamma interferon immunoglobulin G immunoglobulin M antibody interleukin 10 interleukin 7 interleukin 8 interleukin 9 tumor necrosis factor computer assisted tomography coronavirus disease 2019 CRISPR Cas system enzyme linked immunosorbent assay erythrocyte sedimentation rate genotype human limit of detection microarray analysis nucleic acid sequence based amplification open reading frame point of care testing real time reverse transcription polymerase chain reaction recombinase polymerase amplification reverse transcription loop mediated isothermal amplification Review rolling circle amplification Severe acute respiratory syndrome coronavirus 2 virus detection virus genome Western blotting
Page Range: pp. 77-86
Journal or Publication Title: Open Microbiology Journal
Journal Index: Scopus
Volume: 15
Number: 1
Identification Number:
ISSN: 18742858 (ISSN)
Depositing User: مهندس مهدی شریفی

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