Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Design and fabrication of bone tissue scaffolds based on PCL/PHBV containing hydroxyapatite nanoparticles: dual-leaching technique

(2021) Design and fabrication of bone tissue scaffolds based on PCL/PHBV containing hydroxyapatite nanoparticles: dual-leaching technique. J Biomed Mater Res A. pp. 981-993. ISSN 1549-3296

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Design and fabrication of bone tissue scaffolds based on PCLPHBV containing hydroxyapatite nanoparticles dual-leaching technique.pdf

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Abstract

Scaffolds are the important part of the tissue-engineering field that are made from different biomaterials using various techniques. In this study, new scaffold based on polycaprolactone (PCL) and poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV) containing hydroxyapatite nanopraticles (n-HA) were fabricated using the dual-leaching technique (DLT). Morphology, porosity, degradation rate, Fourier transfer infrared ray (FTIR) spectra, surface, and mechanical properties as well as capacity of cell binding and cell proliferation on the constructed scaffolds were evaluated. FTIR analysis showed that n-HA particles have some interest interactions with polymeric chains. The best 3D-structure was seen in PCL70PHBV30 scaffold using the scanning electron microscopy (SEM) and its structure improved in the presence of 3, 5 wt of n-HA. Results of energy dispersive x-ray analysis (EDXA, map of Ca) showed that the nanoparticles have the uniform distribution within the fabricated scaffolds. Porosity analysis showed that the particulate salt leaching technique is a successful approach to building a 3D structure. Increasing of PHBV content and n-HA up to 3 and 5 wt in the PCL matrix led to increase porosity in all samples. Mechanical properties analysis showed that values of compression modulus and strength are decreased with addition of PHBV and HA nanoparticles. These results were directly in line with the results of morphology and porosity. Cell culture experiments demonstrated that the PCL/PHBV/nHA nanocomposite scaffold has a better tendency of proliferation to cells than that of the pure PCL/PHBV scaffold. All of these results suggest promising potentials of the developed PCL/PHBV/nHA scaffolds in this study desire for bone tissue engineering.

Item Type: Article
Keywords: Animals Biocompatible Materials/*chemistry Bone and Bones Cattle Cell Proliferation/drug effects Cells, Cultured Durapatite/*chemistry Mechanical Phenomena Nanocomposites *Nanoparticles Particle Size Polyesters/*chemistry Porosity *Tissue Scaffolds *Pcl *Phbv *bone tissue engineering *hydroxyapatite *nanocomposite scaffold *solvent casting-dual leaching technique
Page Range: pp. 981-993
Journal or Publication Title: J Biomed Mater Res A
Journal Index: Pubmed
Volume: 109
Number: 6
Identification Number: https://doi.org/10.1002/jbm.a.37087
ISSN: 1549-3296
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9412

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