Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Safety of grafting acellular human corneal lenticule seeded with Wharton?s Jelly-Derived Mesenchymal Stem Cells in an experimental animal model

(2021) Safety of grafting acellular human corneal lenticule seeded with Wharton?s Jelly-Derived Mesenchymal Stem Cells in an experimental animal model. Experimental Eye Research. p. 10. ISSN 0014-4835

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Abstract

The present study was conducted to evaluate safety of grafting acellular human corneal lenticule seeded with Wharton?s Jelly-derived Mesenchymal Stem Cells (WJSC) in an experimental animal model. Human corneal lenticules were decellularized with a rate of about 97 with an acceptable lack of cytotoxicity and relatively intact ultrastructure of the lenticules. 12 rabbits underwent unilateral stromal pocketing with implantation of decellularized lenticules. Implantation was performed for 6 rabbits along with graft recellularization with WJSCs. Rabbits were euthanized after 1 month (n = 6) and 3 months (n = 6) to evaluate progression of graft biointegration. No clinical rejection sign was detected during the study. Histopathological analysis showed that, grafts were integrated well with the least distortion of surrounding collagen bundles. After 3 months, labeled WJCS was detected representing viability of stem cells in the host. Increased expression of keratocyte-specific markers showed the potential of recruiting WJSCs as keratocyte progenitor cells to reinforce corneal ultrastructure.

Item Type: Article
Keywords: Cornea Decellularized Graft Lenticule Stem cell Ophthalmology
Page Range: p. 10
Journal or Publication Title: Experimental Eye Research
Journal Index: ISI
Volume: 205
Identification Number: https://doi.org/10.1016/j.exer.2021.108451
ISSN: 0014-4835
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9634

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