Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

A novel label-free electrochemical immunesensor for ultrasensitive detection of LT toxin using prussian blue@gold nanoparticles composite as a signal amplification

(2021) A novel label-free electrochemical immunesensor for ultrasensitive detection of LT toxin using prussian blue@gold nanoparticles composite as a signal amplification. Bioelectrochemistry. p. 9. ISSN 1567-5394

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Abstract

In the current study, a novel electrochemical label-free immunosensor is proposed for sensitive detection of heat-labile enterotoxin (LT) from Escherichia coli. Firstly, a glassy carbon electrode (GCE) was modified by a mixture containing reduced graphene oxide/room temperature ionic liquid (rGO/RTIL) composite. Then, simultaneous electrodeposition of prussian blue and gold nanoparticles led to formation of prus-sian blue@gold nanoparticles (PB@GNPs) composite on the electrode surface. The modified electrode was characterized by field emission scanning electron microscopy (FE-SEM), energy dispersive spec-troscopy (EDS), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. After immobilization of anti-LT and blocking the unreacted sites with BSA (bovine serum albumin), the analytical performance of the proposed immunosensor was evaluated under optimal conditions (i.e. opti-mal pH, incubation time and temperature of incubation). Square wave voltammetry (SWV) was used to determine different concentrations of the LT antigen. The linear dynamic range of the proposed immunosensor was from 0.01 to 50 mg/mL and the detection limit of the immunosensor was found to be 0.0023 mg/mL. An acceptable selectivity in the real sample, long-term stability and good reproducibil-ity made the fabricated immunosensor a good candidate for detecting LT. (c) 2021 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Heat-labile enterotoxin Electrochemical immunosensor Graphen oxide Ionic liquid Gold nanoparticle Prussian blue heat-labile enterotoxin sensitive detection immunosensor graphene nanocomposite biosensor immunoassay electrode films Biochemistry & Molecular Biology Life Sciences & Biomedicine - Other Topics Biophysics Electrochemistry
Page Range: p. 9
Journal or Publication Title: Bioelectrochemistry
Journal Index: ISI
Volume: 142
Identification Number: https://doi.org/10.1016/j.bioelechem.2021.107887
ISSN: 1567-5394
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9646

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