Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae

(2021) Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae. Expert Review of Anti-Infective Therapy. p. 10. ISSN 1478-7210

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Abstract

Background Klebsiella pneumoniae is armed with a wide range of antibiotic resistance mechanisms that mostly challenge effective treatment. The aims of the current study were to identify the clinical strains of K. pneumoniaealso to determine their phenotypes and molecular characterization related to antimicrobial resistance and virulence genes. Research design and methods In this investigation, clinical specimens from different hospitals located in Tehran, Iran, were collected during a nine-month period (December 2018 to August 2019). The K. pneumoniae strains were isolated and identified through standard microbial and biochemical assays. Additionally, disk diffusion, combined disk, Modified Hodge Test (MHT) and PCR were performed for antibiotic resistance and virulence gene analysis, respectively. Results Eighty-four isolates of K. pneumoniae were subjected to the study. According to the combined disk and modified Hodge test results, 27 (52) and 15 pathotypes (62.5) out of resistant strains of isolated K. pneumoniae were detected as ESBL and KPC producers. The virulence genes of mrkD (94) and magA (11) were the highest and lowest among isolates, respectively. Conclusions The high prevalence of antibiotic resistance and virulence genes in conjunction with a significant relationship between the strains revealed a high pathogenic capacity of the isolated pathotypes of K. pneumoniae.

Item Type: Article
Keywords: Klebsiella pneumoniae antibiotic resistance ESBL virulence genes molecular typing modified-hodge test escherichia-coli liver-abscess strains genes association bla(kpc-2) lactamases bla(ndm-1) community Infectious Diseases Microbiology Pharmacology & Pharmacy
Page Range: p. 10
Journal or Publication Title: Expert Review of Anti-Infective Therapy
Journal Index: ISI
Identification Number: https://doi.org/10.1080/14787210.2022.1990040
ISSN: 1478-7210
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/9710

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