Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

The aflatoxin B1 isolating potential of two lactic acid bacteria

(2013) The aflatoxin B1 isolating potential of two lactic acid bacteria. Asian Pacific Journal of Tropical Biomedicine. pp. 732-736. ISSN 22211691 (ISSN)

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Abstract

Objective: To determine lactic acid bacteria's capability to enhance the process of binding and isolating aflatoxin B1 and to utilize such lactic acid bacteria as a food supplement or probiotic products for preventing absorption of aflatoxin B1 in human and animal bodies. Methods: In the present research, the bacteria were isolated from five different sources. For surveying the capability of the bacteria in isolating aflatoxin B1, ELISA method was implemented, and for identifying the resultant strains through 16S rRNA sequencing method, universal primers were applied. Results: Among the strains which were isolated, two strains of Lactobacillus pentosus and Lactobacillus beveris exhibited the capability of absorbing and isolating aflatoxin B1 by respectively absorbing and discharging 17.4 and 34.7 of the aforementioned toxin existing in the experiment solution. Conclusions: Strains of Lactobacillus pentosus and Lactobacillus beveris were isolated from human feces and local milk samples, respectively. And both strains has the ability to isolate or bind with aflatoxin B1. © 2013 Asian Pacific Tropical Biomedical Magazine.

Item Type: Article
Keywords: Aflatoxin Bacteria ELISA test Lactic acid Mycotoxin aflatoxin B1 RNA 16S absorption article bacterial strain bacterium culture bacterium isolation chemical binding controlled study enzyme linked immunosorbent assay Lactobacillus Lactobacillus beveris Lactobacillus pentosus nonhuman priority journal RNA sequence Animals DNA, Bacterial Enzyme-Linked Immunosorbent Assay Feces Humans Milk Polymerase Chain Reaction RNA, Ribosomal, 16S
Divisions:
Page Range: pp. 732-736
Journal or Publication Title: Asian Pacific Journal of Tropical Biomedicine
Journal Index: Scopus
Volume: 3
Number: 9
Identification Number: https://doi.org/10.1016/S2221-1691(13)60147-1
ISSN: 22211691 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/989

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