Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Molecular epidemiology and genetic characterization of Shigella in pediatric patients in Iran

(2018) Molecular epidemiology and genetic characterization of Shigella in pediatric patients in Iran. Infez Med. pp. 321-328. ISSN 1124-9390 (Print) 1124-9390

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Infection with Shigella is considered a major cause of morbidity and mortality in children with diarrhea in developing countries, especially in Iran. Due to the importance of country-level epidemiological data, molecular characterization of genetic determinants of Shigella spp. is a necessity. The aim of the present study was to investigate the prevalence of integron types, bla-CTX-M, bla-SHV and blaTEM beta-lactamase genes of Shigella isolates in pediatric patients in Tehran, Iran. In a time period of 18 months from May 2015 to August 2017, 75 Shigella spp. were isolated from non-duplicative diarrheal stool specimens in six different hospitals in Tehran. The isolates from patients were further analyzed for their antibiotic susceptibility and extended-spectrum beta-lactamase (ESBL) production. Polymerase chain reaction was performed for amplification of the integrons (I, II, III), TEM, SHV, CTX-M15. The prevalence of S. sonnei, S. flexneri, S. dysenteriae and S. boydii were 40 (53.3), 33 (44), 1 (1.3) and 1 (1.3), respectively. The results of an antimicrobial resistance test showed that the high percentage of resistance to nalidixic acid (NA), ampicillin (AMP) and trimethoprim/sulfamethoxazole (SXT) included 38 (50.6), 59 (81.3) and 64 (88) isolates, respectively. Further results revealed that 52 and 76 of Shigella isolates carried intI and intII genes, respectively. In this study, the rates of CTX-M (10.7), SHV (28) and TEM (21.3) were determined, all of which were positive for blaCTX-M15. This study showed the high prevalence of multidrug resistant S. sonnei and S. flexneri. Furthermore, it highlighted the increasing integrons (intI and intII) and ESBL genes, especially blaCTX-M15, in Shigella isolates.

Item Type: Article
Keywords: Anti-Bacterial Agents/pharmacology Child, Preschool Cross-Sectional Studies Dysentery, Bacillary/epidemiology/microbiology Female Humans Integrons Iran/epidemiology Male Microbial Sensitivity Tests Molecular Epidemiology Shigella/drug effects/enzymology/*genetics/isolation & purification beta-Lactamases/genetics
Page Range: pp. 321-328
Journal or Publication Title: Infez Med
Journal Index: Pubmed
Volume: 26
Number: 4
ISSN: 1124-9390 (Print) 1124-9390
Depositing User: مهندس مهدی شریفی

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