Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Rapid screening of toxigenic vibrio cholerae O1 strains from south Iran by PCR-ELISA

(2008) Rapid screening of toxigenic vibrio cholerae O1 strains from south Iran by PCR-ELISA. Iran Biomed J. pp. 15-21. ISSN 1028-852X (Print) 1028-852x

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BACKGROUND: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. METHODS: The 398-bp sequence of a gene that codes for the cholera toxin B subunit was amplified by PCR. The digoxigenin-labeled amplified products were coated on microplates and detected by ELISA. The PCR product was also hybridized with biotin labelled probe and detected by ELISA using streptavidin. RESULTS AND CONCLUSION: The specificity of the PCR was determined using 10 bacterial strains and 50 samples from south Iran. The detection limit was 0.5 pg of the genomic DNA and five bacterial cells. Adaptation of PCR into PCR-ELISA assay format facilitates specific and sensitive detection and diagnosis of human cholera disease. We conclude that this PCR-ELISA is a diagnostic method that specifically detects toxin genes in V. cholera O1 strains. It is more rapid and less cumbersome than other diagnostic methods for detection of toxicity in these strains.

Item Type: Article
Keywords: Enzyme-Linked Immunosorbent Assay/*methods Polymerase Chain Reaction/*methods Sensitivity and Specificity Vibrio cholerae O1/genetics/*isolation & purification/pathogenicity Vibrio cholera Pcr- elisa Immunosorbent assay
Page Range: pp. 15-21
Journal or Publication Title: Iran Biomed J
Journal Index: Pubmed
Volume: 12
Number: 1
ISSN: 1028-852X (Print) 1028-852x
Depositing User: مهندس مهدی شریفی

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