Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

(2019) Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations. Journal of Clinical Microbiology. p. 20. ISSN 0095-1137

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Polymyxins, including polymyxin B and polymyxin E (colistin), are now increasingly being used worldwide to treat patients with multidrug-resistant (MDR) Gram-negative bacterial infections. This necessitates that laboratories employ an accurate and reliable method for the routine performance of polymyxin susceptibility testing. A number of reasons have accounted for the difficulties with susceptibility testing for the polymyxins, including their multicomponent composition, poor diffusion in the agar medium, adsorption to microtiter plates, the lack of a reliable susceptibility test, the lack of a specific breakpoint from professional organizations, the synergistic effect of polysorbate 80, and the development of heteroresistance. This minireview discusses such problems that impact the results of currently available susceptibility testing methods. We also provide emerging concepts on mechanisms of polymyxin resistance, including chromosomally and plasmid-mediated mcr-related resistance. Broad-range investigations on such critical issues in relation to polymyxins can be beneficial for the implementation of effective treatment against MDR Gram-negative bacterial infections.

Item Type: Article
Keywords: antimicrobial susceptibility colistin Gram-negative bacteria polymyxin resistance resistant acinetobacter-baumannii gram-negative bacilli in-vitro activity pseudomonas-aeruginosa carbapenem-resistant klebsiella-pneumoniae agar dilution colistin susceptibility enterobacter-cloacae broth microdilution Microbiology
Page Range: p. 20
Journal or Publication Title: Journal of Clinical Microbiology
Journal Index: ISI
Volume: 57
Number: 4
Identification Number:
ISSN: 0095-1137
Depositing User: مهندس مهدی شریفی

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