Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Diisopropyl-fluorophosphatase as a catalytic bioscavenger

(2016) Diisopropyl-fluorophosphatase as a catalytic bioscavenger. Journal of Applied Biotechnology Reports. pp. 477-482. ISSN 23221186 (ISSN)

[img] Text
Diisopropyl-fluorophosphatase as a catalytic bioscavenger.pdf

Download (564kB)

Official URL:


Organophosphorus chemicals, used as pesticides and warfare nerve agent, are highly toxic compounds that inhibit acetylcholine esterase enzyme rapidly. A novel effective treatment for nerve gas poising is using of hydrolytic enzymes to degradation of these agents. Diisopropyl-fluorophosphatase (DFPase) from Loligo vulgaris is highly stable and robust biocatalyst for the hydrolysis of various chemical warfare agents such as sarin, soman, tabun. Unfortunately, wild-type DFPase prefers less toxic isomers of these agents leading to slower detoxification. Also, due to non-human origin of the enzyme, immunological reactions occur when it is injected into body. In order to using DFPase as in vivo detoxifying agent, some manipulations to augment of its efficiency and to decrease of immunogenic problems are needed. Modifications such as PEGylation is one of the possible solutions to conquer these problems. Engineering of the enzyme for creating of new efficient variants is an interesting research field which leads to occurrence of novel and prominent bioscavenger, and delivery of these functional molecules to circulation in order to enzymatic hydrolysis of toxic agents would be the final object of research efforts. © 2016, Baqiyatallah university of medical sciences. All rights reserved.

Item Type: Article
Keywords: Bioscavenger DFPase Nerve agents Organophosphorus compounds
Page Range: pp. 477-482
Journal or Publication Title: Journal of Applied Biotechnology Reports
Journal Index: Scopus
Volume: 3
Number: 4
ISSN: 23221186 (ISSN)
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item