Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Curcumin Mitigates Radiation-induced Lung Pneumonitis and Fibrosis in Rats

(2018) Curcumin Mitigates Radiation-induced Lung Pneumonitis and Fibrosis in Rats. International Journal of Molecular and Cellular Medicine. pp. 212-219. ISSN 2251-9637

[img] Text
Curcumin mitigates radiation-induced lung pneumonitis and fibrosis in rats.pdf

Download (649kB)

Official URL:


Radiation-induced lung injury is one of the most prominent factors that interfere with chest cancer radiotherapy, and poses a great threat to patients exposed to total body irradiation. Upregulation of pro-oxidant enzymes is one of the main mechanisms through which the late effects of ionizing radiation on lung injury can be exerted. Interleukin (IL)-4 and IL-13 are two important cytokines that have been proposed to be involved in this process. Through stimulation of dual oxidase 1 and 2 (DUOX 1 & 2), they induce chronic oxidative stress in irradiated tissues. In this study, we evaluated the effects of curcumin treatment on the regulation of IL-4 and IL-13, DUOX1 & 2 genes as well as the pathological changes developed by this treatment. Twenty male Wistar rats were divided into four groups: radiation only; curcumin only; radiation + curcumin; and control group with neither pharmacotherapy nor radiation. Curcumin was administered for 4 and 6 consecutive days before and after irradiation, respectively. Also, the chest area was irradiated with 15 Gy using a cobalt-60 gamma rays source. All rats were sacrificed 67 days after irradiation, followed by the assessment of the levels of IL-4 and IL-13; the expression of IL-4 receptor-a1 (IL4Ra1), IL13Ra2, DUOX1 and DUOX2, and finally the histopathological changes were evaluated. Radiation led to the increased level of IL-4, while the level of IL-13 showed no change. QPCR results showed the upregulation of IL4Ra1, DUOX1 and DUOX2 following lung irradiation. Histopathological evaluation also showed a remarkable increase in pneumonitis and fibrosis. Treatment with curcumin downregulated the expression of IL-4, IL4Ra1, DUOX1 & 2. Furthermore, it could mitigate pneumonitis and fibrosis following lung irradiation. The late effects of radiation-induced lung injury may be due to the upregulation of DUOX1 & 2 genes. Curcumin, through modulation of these genes, may contribute to the protection against ionizing radiation.

Item Type: Article
Keywords: Curcumin radiation radioprotection DUOX1 DUOX2 pulmonary-fibrosis clinical-trial chemoprevention antioxidant mechanisms toxicity protects cells Research & Experimental Medicine
Page Range: pp. 212-219
Journal or Publication Title: International Journal of Molecular and Cellular Medicine
Journal Index: ISI
Volume: 7
Number: 4
Identification Number:
ISSN: 2251-9637
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item