Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

rpoB Gene Sequencing for Identification of Rapidly Growing Mycobacteria

(2017) rpoB Gene Sequencing for Identification of Rapidly Growing Mycobacteria. Archives of Pediatric Infectious Diseases. p. 4. ISSN 2322-1828

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Background: Rapidly growing mycobacteria (RGM) are increasingly recognized as a cause of human infections. Rapid and reliable identification of RGM at species level should be carried out as a means of effective patient managements. Methods: Twenty clinical samples of RGM isolated from suspected tuberculosis (TB) patients were included. Different phenotypic tests and a hsp65-PCR restriction analysis (PRA) method were used to identify the isolated organisms to species level. Sequence analysis of the rpoB gene was also used for molecular identification of clinical isolates. Results: Phenotypic evaluation of clinical isolates assigned 19 (95) isolates of RGM to M. fortuitum complex. Using hsp65-PRA, 13 isolates of M. fortuitum complex were identified as M. fortuitum, 4 isolates as M. abscessus and 1 isolates as M. chelonae. However, two isolates had identical hsp65-PRA patterns; one was indistinguishable from M. conceptionense and M. senegalense and another was indistinguishable from M. peregrinum and M. porcinum. By the rpoB gene sequence analysis, all species studied were readily discriminated from each other. Conclusions: rpoB gene sequencing has a high discriminatory power, which easily permits the identification of clinical isolates of RGM to the species level. It unambiguously differentiates between closely related species with restricted biochemical and PRA differences. This procedure is suggested as a first-line identification method for RGM.

Item Type: Article
Keywords: Mycobacterium Sequencing Iran rpoB Gene restriction enzyme analysis nontuberculous mycobacteria infections diagnosis samples epidemiology disease Pediatrics
Page Range: p. 4
Journal or Publication Title: Archives of Pediatric Infectious Diseases
Journal Index: ISI
Volume: 5
Number: 2
Identification Number:
ISSN: 2322-1828
Depositing User: مهندس مهدی شریفی

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