Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization

(2017) Histologic analyses of different concentrations of TritonX-100 and Sodium dodecyl sulfate detergent in lung decellularization. Cellular and Molecular Biology. pp. 46-51. ISSN 0145-5680

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Pulmonary diseases cusecs a large portion of mortality in the world. There is no more cure for pulmonary diseases and many approaches are needed for finding ways to cure. Nowadays, implantation and drugs are only ways for curing those people who are facing with these diseases. Tissue engineering and regenerative medicine have been appeared as multidisciplinary field and also, they presents new therapeutic approaches for pulmonary diseases. One of these therapeutic approaches is decellularization which removes cellular but leaves intact important extracellular matrix (ECM) proteins and three-dimensional (3D) architecture and also, this approach has been studied for in-vitro and ex-vivo. In this study, we aimed to investigate a comparison of different concentrations of Triton X-100 and Sodium dodecyl sulfate detergents in lung decellularization in order to evaluate the effects of different concentrations and times of mentioned detergents on three dimensional and ECM proteins lung. Two detergents (Triton-X100 and Sodium dodecyl sulfat) were used with different concentrations for decellularizing rat lungs for maintaining of three-dimensional lung architecture and ECM protein compositions which have significant roles in differentiation and migration of stem cells. Results showed that SDS 0.05, 0.1 and Triton-X100 0.1 could maintain 3D, elastin and collagen better than other concentrations in 24 and 48 h-decellularization. We concluded that these approaches can help to achieve three-dimensional architecture and extracellular matrix of lung with minimum destruction for next step such as recellularization and in-vivo study.

Item Type: Article
Keywords: Rat lung Decellularization SDS Triton X-100 stem-cell differentiation regenerative medicine de-cellularization in-vitro matrix scaffolds recellularization implantation tissues organs Biochemistry & Molecular Biology Cell Biology
Page Range: pp. 46-51
Journal or Publication Title: Cellular and Molecular Biology
Journal Index: ISI
Volume: 63
Number: 7
Identification Number:
ISSN: 0145-5680
Depositing User: مهندس مهدی شریفی

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