Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Peroxisome proliferator-activated receptor (PPAR)-γ modifies Aβ neurotoxin-induced electrophysiological alterations in rat primary cultured hippocampal neurons

(2019) Peroxisome proliferator-activated receptor (PPAR)-γ modifies Aβ neurotoxin-induced electrophysiological alterations in rat primary cultured hippocampal neurons. Iranian Journal of Pharmaceutical Research. pp. 1403-1418. ISSN 17350328 (ISSN)

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Alzheimer’s disease (AD) is undoubtedly one of the serious and growing public health challenges in the world today. There is an unmet need for new and effective preventative and therapeutic treatment approaches for AD, particularly at early stages of the disease. However, the underlying mechanism against Aβ-induced electrophysiological alteration in cultured hippocampal pyramidal neurons is still not fully understood. This study investigated the impacts of activation and inhibition of PPAR-γ/δ on the Aβ-induced functional toxicity, which occured before cell death, using patch clamp technique. Findings demonstrated that Aβ treatment alone altered the normal electrophysiological properties and reduced the Ca2+ channel currents in primary cultured hippocampal pyramidal neurons without any major changes either in cell structure, as evidenced by electron microscope examination, or cell viability. Rosiglitazone (30 µM), a potent PPAR-γ activator, when co-treated with Aβ (100 nM) prevented almost completely the induction of function toxicity of Aβ, as evidentiated by restored normal appearing electrophysiological properties. Inhibition of PPAR-γ/δ by FH535 (15 µM), an inhibitor of both Wnt/beta-catenin signaling and PPAR-γ and δ activity, when applied in combination of Aβ not only worsen the toxic electrophysiological effects of Aβ on firing frequency, membrane resistance and cell viability, but also even preserved the suppressive effect of Aβ on Ca2+ channel current when compared to control condition. Overall, these findings suggest that PPAR-γ activation could be a potential candidate to prevent the functional changes induced by low concentration of Aβ which may possibly occur in neurons during early stages of AD. © 2019, Iranian Journal of Pharmaceutical Research. All rights reserved.

Item Type: Article
Keywords: Alzheimer’s disease Amyloid Beta (Aβ) Ca2+ channel current Hippocampal pyramidal neurons Neurodegenerative diseases PPAR-γ amyloid beta neurotoxin amyloid beta protein calcium ion neurotoxin peroxisome proliferator activated receptor gamma reactive oxygen metabolite rosiglitazone unclassified drug animal cell Article cell proliferation cell structure cell viability controlled study electron microscopy electrophysiology half-activation voltage hippocampal neuronal culture in vitro study lysosome mathematical model membrane depolarization membrane potential membrane resistance microscopy mitochondrion MTS assay nerve cell degeneration nerve cell excitability nonhuman osmolarity patch clamp technique rat Wnt signaling
Page Range: pp. 1403-1418
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Journal Index: Scopus
Volume: 18
Number: 3
Identification Number:
ISSN: 17350328 (ISSN)
Depositing User: مهندس مهدی شریفی

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