Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Antimicrobial effects of Ferula gummosa Boiss gum against extended-spectrum beta-lactamase producing Acinetobacter clinical isolates

(2016) Antimicrobial effects of Ferula gummosa Boiss gum against extended-spectrum beta-lactamase producing Acinetobacter clinical isolates. Iranian Journal of Microbiology. pp. 263-273. ISSN 2008-3289

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Antimicrobial effects of Ferula gummosa boiss gum against extended-spectrum $β$-lactamase producing Acinetobacter clinical isolates.pdf

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Background and Objectives: Acinetobacter spp. are important causes of nosocomial infections. They possess various antibiotic resistance mechanisms including extended spectrum beta lactamases (ESBLs). The aim of this study was to determine antibiotic resistance profile of Acinetobacter clinical isolates especially among ESBL-producing strains and to investigate the antimicrobial effects of oleo-gum-resin extract and essential oil of Ferula gummosa Boiss. Materials and Methods: 120 Acinetobacter strains were isolated from various clinical samples of hospitalized patients in Baqiyatallah hospital, Tehran during 2011-2012. Antibiotic susceptibility test was performed on the isolates using disk diffusion method. To detect and confirm the ESBL-positive isolates, phenotypic and genotypic tests were performed. Three types of F. gummosa oleo-gum-resin extracts and essential oils were prepared and the bioactive components of F. gummosa Boiss extracts were determined by GC-Mass chromatography. F. gummosa antimicrobial activity was evaluated against standard strain of Acinetobacter baumannii (ATCC19606) as well as Acinetobacter clinical isolates using well and disk diffusion methods. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution method. Results: 46 isolates were resistant to all tested antibiotics. All clinical isolates were resistant to cefotaxime. 12.94 of the isolates were phenotypically ESBL-producing among which 94.2 carried ESBL genes (bla(PER-1), bla(OXA-4) and bla(CTX-M)) detected by PCR. Oleo-gum-resin of F. gummosa had significant antibacterial activity and alcoholic essential oil had higher inhibitory effect on Acinetobacter strains (MIC of 18.75 mg/ml). Conclusion: Ferula gummosa extract contained components with well-known antimicrobial effects.

Item Type: Article
Keywords: Acinetobacter spp. Antibiotic resistance Antimicrobial activity ESBLs Ferula gummosa boiss Microbiology
Page Range: pp. 263-273
Journal or Publication Title: Iranian Journal of Microbiology
Journal Index: ISI
Volume: 8
Number: 4
ISSN: 2008-3289
Depositing User: مهندس مهدی شریفی

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