Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins

(2016) Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins. Microbes and Infection. pp. 421-429. ISSN 1286-4579

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Infectious diarrhoea remains an emerging problem in the world health program. Among diarrheagenic agents, Vibrio cholerae and enterotoxigenic and enterohemorrhagic Escherichia coli are critical enteropathogens. AB5 toxin produced by these bacteria, heat-labile enterotoxin (LT), cholera enterotoxin (CT), and shiga-like cytotoxin (STX) can target the immune system and are subunit vaccine candidates. A chemically-synthesized chimeric construct composed of the binding subunits of these toxins (LTB, STXB, and CTXB) was developed based on bioinformatics studies. The whole chimeric protein (rLSC) and each of the segments (rLTB, rSTXB, and rCTXB) were expressed in a prokaryotic expression system (E. coli), purified, and analysed for their immunogenic properties. The results indicate that these recombinant proteins were effectively able to present appropriate epitopes to an animal model of the immune system which could result in and increase IgG in serum and immune responses that protect against the binding activity of these toxins. The immunological assays revealed that the sera of immunized mice prevented toxins from binding to their specific receptors and neutralized their toxic effects. The proposed construct should be considered as a potent immunogen to prevent toxicity and diarrhoea. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Item Type: Article
Keywords: Diarrhoea ETEC EHEC Vibrio cholerae Recombinant protein Subunit vaccine heat-labile-enterotoxin escherichia-coli o157h7 cholera-toxin neutralizing antibodies chimeric protein child-mortality vibrio-cholerae shiga toxin-2 vaccine expression Immunology Infectious Diseases Microbiology
Page Range: pp. 421-429
Journal or Publication Title: Microbes and Infection
Journal Index: ISI
Volume: 18
Number: 6
Identification Number:
ISSN: 1286-4579
Depositing User: مهندس مهدی شریفی

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