Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation

(2016) Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation. Reproductive Biology and Endocrinology. p. 8.

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Background: Although routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men. Methods: Semen samples were collected from 25 asthenozoosprmic men and divided into the following groups (n = 5/group): fresh semen (control); frozen-thawed semen without treatment; frozen-thawed semen with NGF treatment (0.5, 1, and 5 ng/ml). Prior to dividing the asthenozoospermic samples, 200 mu l of each sample was collected for NGF content assessment by ELISA and then compared with normozoospermic semen samples (25 normozoospermic men). Sperm motility and viability were assessed according to WHO criteria. Furthermore, intracellular nitric oxide and DNA fragmentation were evaluated by Flow Cytometry. Results: NGF content was significantly higher in normozoospermic compared with asthenozoospermic men. Cryopreservation of asthenozoospermic semen samples significantly decreased sperm viability and motility, and increased intracellular nitric oxide concentration and DNA damage (p < 0.01). In asthenozoospermic frozen-thawed samples treated with 0.5 ng/ml exogenous NGF, we observed a significantly increased viability, motility, and decreased DNA fragmentation (p < 0.05), but intracellular nitric oxide concentration was not reduced. The other high doses (1 and 5 ng/ml) had no significant effect on the variables. Conclusion: Supplementation with exogenous NGF could have partial and limited protective effect during cryopreservation of human spermatozoa but further research is needed to evaluate the possible clinical applications.

Item Type: Article
Keywords: Nerve growth factor Cryopreservation Nitric oxide DNA fragmentation Human spermatozoa nitric-oxide production factor ngf embryo development human spermatozoa oxidative stress dna-damage apoptosis rat motility cells Endocrinology & Metabolism Reproductive Biology
Page Range: p. 8
Journal or Publication Title: Reproductive Biology and Endocrinology
Journal Index: ISI
Volume: 14
Identification Number:
Depositing User: مهندس مهدی شریفی

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