Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Bacteriophage-based biosensor for detection of E. Coli bacteria on graphene modified carbon paste electrode

(2019) Bacteriophage-based biosensor for detection of E. Coli bacteria on graphene modified carbon paste electrode. Nanoscience and Nanotechnology - Asia. pp. 408-413. ISSN 22106812 (ISSN)

Full text not available from this repository.

Official URL:


Background: Escherichia coli (E. coli) bacteria is one of the hazardous human pathogens. Consequently, developing the rapid and effective method for identification and quantization of E. coli is popular in biotechnological researches in recent years. Experimental: In this research, a label-free capacitance E. coli biosensor was fabricated based on immobilizing bacteriophage on the carbon paste electrode (Cp). Reduced graphene (RGr) was synthesized and used as a substrate for immobilization of bacteriophage on the Cp surface. E. coli bacteriophage was trapped in graphene modified carbon paste electrodes. The immobilization accuracy was confirmed via electrochemical techniques. The modified electrodes were applied as indicator electrodes for capacitance measurements of E. coli. Results: Through this method, E. coli was detected in a concentration range of 33×10-3 to 330×10-3 N L-1 (number of E. coli per Liter) with a correlation coefficient of 0.99 and a detection limit of 12×10-3 N L-1. Conclusion: The proposed biosensor has a fast response time of about 5 s and good selectivity over other bacteria. © 2019 Bentham Science Publishers.

Item Type: Article
Keywords: Bacteriophage Biosensor Cell culture E. Coli bacteria Electrode Microorganisms Bacteriophages Biosensors Capacitance Capacitance measurement Electrodes Graphene Carbon paste electrode Concentration ranges Correlation coefficient E. coli Electrochemical techniques Escherichia coli (E. coli) Indicator electrodes Modified carbon paste electrode Escherichia coli
Page Range: pp. 408-413
Journal or Publication Title: Nanoscience and Nanotechnology - Asia
Journal Index: Scopus
Volume: 9
Number: 3
Identification Number:
ISSN: 22106812 (ISSN)
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item