Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Diagnosis of Mesobuthus eupeus envenomation by skin test: Reverse passive Arthus reaction

(2014) Diagnosis of Mesobuthus eupeus envenomation by skin test: Reverse passive Arthus reaction. Toxicon. pp. 133-140. ISSN 0041-0101

[img] Text
Diagnosis of Mesobuthus eupeus envenomation by skin test Reverse passive Arthus reaction.pdf

Download (2MB)

Official URL:


While being stung by two large families of scorpions, Buthidae and Scorpionidae have different symptoms and complications, a similar maintenance treatment usually considers as the scorpion species could not be identified easily. Therefore, this study was an attempt to develop an immunologic response for designing a skin sensitivity test that can be used to determine the poisoning. The sensitivity and the specificity of RPA reaction for detecting experimental envenomated mice were evaluated. The inflammatory response for detection of envenomation was obtained by the injection of a solution containing complement, polyelectrolytes and purified monovalent antibodies. As the result, 84.44 sensitivity and 100 specificity recorded 15 min after challenge. Macroscopic findings were also confirmed histologically. No cross-reactions were observed with other species of scorpions and snake venoms. Designed Skin test induced obvious inflammatory reaction without any histological lesions. Besides adding the complement components and polyelectrolyte to the monovalent antibody leads to an increased susceptibility of inflammatory cells in this reaction, resulting in forming a visible inflammation in a short time. According to satisfactory specificity and sensitivity and visible results in about 15 min, non-harmful and cost benefity of reverse passive Arthus test can be used for diagnosis of scorpion envenomation. (C) 2013 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: Envenomation Scorpion Diagnosis Reverse passive Arthus mediated tissue-injury immune-complex mast-cells venom antigens hypersensitivity involvement vasculitis invivo drugs Pharmacology & Pharmacy Toxicology
Page Range: pp. 133-140
Journal or Publication Title: Toxicon
Journal Index: ISI
Volume: 77
Identification Number:
ISSN: 0041-0101
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item