Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Anti-inflammatory effects of serum isolated from animals on intermittent feeding in C6 glioma cell line

(2011) Anti-inflammatory effects of serum isolated from animals on intermittent feeding in C6 glioma cell line. Neuroscience Letters. pp. 32-35. ISSN 0304-3940

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Multiple sclerosis (MS) is a demyelinating disease of the CNS. Early inflammation leads to later destruction of myelin in MS. Dietary restriction (DR) produces anti-inflammatory and immunomodulatory effects in many species. Based on the reported anti-inflammatory effects of DR, we investigated whether sera collected from rats fed on intermittent feeding (IF, a type of DR) diet could modulate cytokine secretion and matrix metalloproteinase (MMP-2) activity that are involved in MS pathogenesis. Cytokine levels (IL-6 and TGF-beta 1) were measured in supernatant from C6 glioma cell line cultures treated with IF and AL fed animals' sera by enzyme-linked immunosorbent assay (ELISA) and MMP-2 activity was detected by gelatin zymography. Our results indicated that sera of animals on IF diet significantly reduced IL-6 (p <0.05) and increased TGF-beta 1 (p <0.05) production by C6 glioma cells. A significant decrease (p <0.05) in MMP-2 activity was also found. These results indicate anti-inflammatory and immunomodulatory activity in the sera of animals on IF regimen on cells involved in multiple sclerosis pathogenesis. Further studies on the detection of factors responsible for such activities and their mechanism of action in MS pathogenesis are recommended. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Keywords: Multiple sclerosis Intermittent feeding Cytokines Inflammation MMP-2 experimental autoimmune encephalomyelitis multiple-sclerosis matrix-metalloproteinase caloric restriction mononuclear-cells transforming growth-factor-beta-1 il-6-deficient mice oxidative stress interferon-gamma immune function Neurosciences & Neurology
Page Range: pp. 32-35
Journal or Publication Title: Neuroscience Letters
Journal Index: ISI
Volume: 487
Number: 1
Identification Number:
ISSN: 0304-3940
Depositing User: مهندس مهدی شریفی

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