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Baqiyatallah University of Medical Sciences

Nucleic acid isothermal amplification technologies - A review

(2008) Nucleic acid isothermal amplification technologies - A review. Nucleosides Nucleotides & Nucleic Acids. pp. 224-243. ISSN 1525-7770

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Nucleic acid amplification technologies are used in the field of molecular biology and recombinant DNA technologies. These techniques are used as leading methods in detecting and analyzing a small quantity of nucleic acids. The polymerase chain reaction (PCR) is the most widely used method for DNA amplification for detection and identification of infectious diseases, genetic disorders and other research purposes. However, it requires a thermocycling machine to separate two DNA strands and then amplify the required fragment. Novel developments in molecular biology of DNA synthesis in vivo demonstrate the possibility of amplifying DNA in isothermal conditions without the need of a thermocycling apparatus. DNA polymerase replicates DNA with the aid of various accessory proteins. Recent identification of these proteins has enabled development of new in vitro isothermal DNA amplification methods, mimicking these in vivo mechanisms. There are several types of isothermal nucleic acid amplification methods such as transcription mediated amplification, nucleic acid sequence-based amplification, signal mediated amplification of RNA technology, strand displacement amplification, rolling circle amplification, loop-mediated isothermal amplification of DNA, isothermal multiple displacement amplification, helicase-dependent amplification, single primer isothermal amplification, and circular helicase-dependent amplification. In this article, we review these isothermal nucleic acid amplification technologies and their applications in molecular biological studies.

Item Type: Article
Keywords: PCR TMA NASBA SMART SDA RCA LAMP IMDA HDA SPIA cHDA strand-displacement amplification whole-genome amplification linked-immunosorbent-assay in-vitro amplification neisseria-gonorrhoeae chlamydia-trachomatis molecular beacons urine specimens dna-polymerase rna Biochemistry & Molecular Biology
Page Range: pp. 224-243
Journal or Publication Title: Nucleosides Nucleotides & Nucleic Acids
Journal Index: ISI
Volume: 27
Number: 3
Identification Number:
ISSN: 1525-7770
Depositing User: مهندس مهدی شریفی

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