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Baqiyatallah University of Medical Sciences

Paraoxon inhibits GABA uptake in brain synaptosomes

(2007) Paraoxon inhibits GABA uptake in brain synaptosomes. Toxicology in Vitro. pp. 1499-1504. ISSN 0887-2333

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To investigate possible effect of paraoxon (10(-9)-10(-3) M) on GABA uptake, we used rat cerebral cortex synaptosomes. K(M) and V(max) of GABA uptake were determined in presence of paraoxon (10(-3) M). Acetylcholine and its antagonists (atropine and mecamylamine) were used for evaluating cholinergic-dependency of uptake. Type of transporter involved was determined by using glial (beta-alanine) and neuronal (DABA) GABA uptake inhibitors. The results of the study showed that paraoxon at low doses (10(-9)-10(-6) M) increased and at high doses (10(-5-)-10(-3) M) decreased GABA uptake. One millimolar paraoxon significantly decreased V(max) (175.2 +/- 4.23 vs. 80.4 +/- 2.03, P < 0.001) of GABA uptake while had no effect on its K(m). DABA significantly decreased GABA uptake (P < 0.001) while beta-alanine had no effect. In conclusion, present data suggests that paraoxon probably acts as non-competitive antagonist of GABA uptake. (C) 2007 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: paraoxon synaptosome GABA uptake V(max) K(m) gamma-aminobutyric-acid cerebral-cortex induced seizure glutamate release cholinesterase rats mechanisms transport epilepsy Toxicology
Page Range: pp. 1499-1504
Journal or Publication Title: Toxicology in Vitro
Journal Index: ISI
Volume: 21
Number: 8
Identification Number:
ISSN: 0887-2333
Depositing User: مهندس مهدی شریفی

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