Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Book #7343

Afsharpaiman, S. and Mamishi, S. and Purakbari, B. and Abbasi, A. (2007) Diagnosis of bacterimia in febrile children with using universal PCR in comparing blood culture. 5th European Congress on Tropical Medicine and International Health . Medimond S R L, 40128 Bologna, 95-+. ISBN 978-88-7587-356-1

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The objective of this study is to investigate the prevalence of bacteremia in febrile pediatric parents, comparing universal PCR and conventional blood culture. One hundred consecutive febrile children (45 males, 55 females) with suspected septicemia were evaluated. A total of 100 paired blood samples were collected from children to analyze for bacterial detection using universal PCR and culture. Twelve patients were blood culture positive. The most common pathogen isolated from blood Culture wasa Staphylococcus aurous. The PCR was positive in 19 patients. The comparison revealed sensitively, specificity and accuracy of 91.67, 90.91 and 91, respectively, for PCR. The present study shows that the use of PCR is more sensitive than the use of conventional blood techniques for the detection of bacterium pathogens based on patients' clinical context.

Item Type: Book
Keywords: Bacteremia sepsis polymerase chain reaction (PCR) 16S rRNA polymerase-chain-reaction ribosomal-rna gene amplification septicemia bacteremia assay dna
Page Range: 95-+
Journal Index: ISI
Publisher: Medimond S R L
ISBN: 978-88-7587-356-1
Depositing User: مهندس مهدی شریفی

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