Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Inhibition of herpes simplex virus type 1 by copper oxide nanoparticles

(2020) Inhibition of herpes simplex virus type 1 by copper oxide nanoparticles. Journal of Virological Methods. p. 7. ISSN 0166-0934

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There are accumulating reports of the emergence of drug-resistant strains of HSV-1 that have become a barrier to successful treatment of HSV-1 infection. Therefore, there is a pressing need to identify and evaluate alternative antiherpetic agents. The aim of the present study was to investigate the effect of copper oxide nanoparticles (CuO-NPs) on HSV-1 infection. The MTT assay was applied to examine the cytotoxic effects of CuO-NPs on Vero cells. Antiherpetic potency was determined using the TCID50 and quantitative Real-Time PCR assays. To evaluate the inhibitory impact of CuO-NPs on the expression of viral antigens, an indirect immunofluorescence assay (IFA) was performed. Acyclovir was used as a reference drug in all experiments. Exposure of HSV-1 with CuO-NPs at the highest non-toxic concentration (100 mu g/mL) resulted in 2.8 1og10 TCID50 reduction in infectious virus titer as compared with virus control (P < 0.0001). This concentration of CuO-NPs was associated with 83.3 inhibition rate, which was estimated based on the HSV-1 viral load compared to virus control. Our findings demonstrated that CuO-NPs are associated with a significant antiviral potency against HSV-1. This feature shows strong potential for CuO-NPs to be used in topical formulations for the treatment of orolabial or genital herpetic lesions.

Item Type: Article
Keywords: Copper oxide Nanoparticle Antiviral activity Herpes simplex virus inactivation surfaces cuo Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Virology
Page Range: p. 7
Journal or Publication Title: Journal of Virological Methods
Journal Index: ISI
Volume: 275
Identification Number:
ISSN: 0166-0934
Depositing User: مهندس مهدی شریفی

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