Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Synthesis, characterization and DNA binding studies of a new ibuprofen-platinum(II) complex

(2020) Synthesis, characterization and DNA binding studies of a new ibuprofen-platinum(II) complex. J Biomol Struct Dyn. pp. 1119-1129. ISSN 0739-1102

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The study is focused on the synthesis of a novel complex of ibuprofen and Platinum(II). The formation of the product was characterized through analytical tools including Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance, ultraviolet-visible spectroscopy, mass spectrometry as well as density functional theory. Using the continuous variation method, the stoichiometry of Pt(HIb)(2)(Cl)(2) binding on DNA (ct-DNA) determines as a single class of binding. Based on the results of Stern-Volmer analysis on the fluorescence quenching data, the quenching mechanism was determined to be static in nature. The studies indicated that the complex could bind DNA molecules via groove binding for four major reasons. Initially, the complex-DNA binding constant determined based on spectrophotometric data were found to be comparable to those of groove-binding drugs. In addition, the competitive fluorimetric based on the applications of Hoechst 33258 proved the ability of Pt(HIb)(2)(Cl)(2) to displace with Hoechst in its DNA-bounded form, reflecting the competition between Pt(HIb)(2)(Cl)(2) and Hoechst for groove binding. Further, no considerable changes were observed in the intensity of the methylene blue (MB)-DNA system after adding the Pt(HIb)(2)(Cl)(2) complex, reflecting the stability of MB molecules in the DNA helix and a non-intercalative bonds of Pt(HIb)(2)(Cl)(2) interaction on DNA. Finally, minor changes in the viscosity of DNA in the presence of Pt(HIb)(2)(Cl)(2), indicated that changes in the length of DNA in the presence of the complex are negligible, supporting the assumption of DNA groove-binding. Also induced CD spectral changes and docking simulations were in favor of the groove mechanism for the Pt(HIb)(2)(Cl)(2)-DNA binding.Communicated by Ramaswamy H. Sarma.

Item Type: Article
Keywords: Algorithms *Chemistry Techniques, Synthetic DNA/*chemistry/metabolism Density Functional Theory Ibuprofen/*chemistry Macromolecular Substances/chemical synthesis/*chemistry Models, Theoretical Molecular Conformation Molecular Docking Simulation Molecular Structure Platinum/*chemistry Protein Binding Spectrum Analysis Static Electricity Structure-Activity Relationship Thermodynamics Viscosity *DFT analysis *DNA interaction *Pt(HIb)2(Cl)2 *groove binding *ibuprofen drug
Page Range: pp. 1119-1129
Journal or Publication Title: J Biomol Struct Dyn
Journal Index: Pubmed
Volume: 38
Number: 4
Identification Number:
ISSN: 0739-1102
Depositing User: مهندس مهدی شریفی

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