Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Rapid diagnosis of Neisseria meningitides by PCR method

(2006) Rapid diagnosis of Neisseria meningitides by PCR method. Yakhteh. 92-97+157. ISSN 15614921 (ISSN)

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Introduction: The aim of this study was to setup, optimize and introduce a sensitive and specific PCR detection method for identification of Neisseria meningitidis DNA in clinical samples. Material and Methods: Capsular transport gene A (ctrA) was selected as a specific target sequence. This primer pair amplifies 101bp of the target gene. Neisseria meningitidis strain: ATCC; 13090 and Neisseria meningitides serogroup C were used as a standard organism for optimization experiments. A range of bacterial pathogens were used for specificity testing, including, Haemophilus influenzae Type b: ATCC; 49766, Escherichia coli: ATCC;35218, Enterobacter, Klebsiella pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus group D. Phenol - chloroform method was used for DNA extraction. Amplified product was detected by gel agarose electrophoresis, stained by ethidiome bromide. Results: Our results confirmed amplification of the expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg of Neisseria meningitidis DNA as a final detection limit. Conclusion: As a conclusion, PCR is a method with high sensivity and specificity and specificity which can be performed within 3 hours, and therefore utilization of this test in the clinical laboratories can help rapid diagnosis of Neisseria meningitides in clinical samples.

Item Type: Article
Keywords: ctrA gene Meningitis Neisseria meningitidis PCR article DNA extraction Enterobacter Escherichia coli gene sequence Haemophilus influenzae type b Klebsiella pneumoniae nonhuman polymerase chain reaction Staphylococcus aureus Streptococcus group D Streptococcus pneumoniae
Page Range: 92-97+157
Journal or Publication Title: Yakhteh
Journal Index: Scopus
Volume: 8
Number: 30
ISSN: 15614921 (ISSN)
Depositing User: مهندس مهدی شریفی

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