Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Species-specific PCR for the Diagnosis and Determination of Antibiotic Susceptibilities of Brucella Strains Isolated from Tehran, Iran

(2016) Species-specific PCR for the Diagnosis and Determination of Antibiotic Susceptibilities of Brucella Strains Isolated from Tehran, Iran. Iran J Pathol. pp. 238-247. ISSN 1735-5303 (Print) 1735-5303

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/27799972

Abstract

BACKGROUND: Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of Brucella at the species level and determining the antibiotic susceptibility pattern of Brucella in Iran. METHODS: Sixty-eight Brucella specimens (38 animal and 30 human specimens) were analyzed using PCR (using one pair of primers). Antibiotic susceptibility patterns were evaluated and compared using the E-Test and disk diffusion susceptibility test. Tigecycline susceptibility pattern was compared with other antibiotics. RESULTS: Thirty six isolates of B. melitensis, 2 isolates of B. abortus and 1 isolate of B. suis from the 38 animal specimens, 24 isolates of B. melitensis and 6 isolates of B. abortus from the 30 human specimens were differentiated. The MIC50 values of doxycycline for human and animal specimens were 125 and 10 mug/ml, respectively, tigecycline 0.064 mug/ml for human specimens and 0.125mug/ml for animal specimens, and trimethoprim/ sulfamethoxazole and ciprofloxacin 0.065 and 0.125mug/ml, respectively, for both human and animal specimens. The highest MIC50 value of streptomycin in the human specimens was 0.5mug/ml and 1mug/ml for the animal specimens. The greatest resistance shown was to tetracycline and gentamicin, respectively. CONCLUSION: Uniplex PCR for the detection and differentiation of Brucella at the strain level is faster and less expensive than multiplex PCR, and the antibiotics doxycycline, rifampin, trimethoprim-sulfamethoxazole, ciprofloxacin, and ofloxacin are the most effective antibiotics for treating brucellosis. Resistance to tigecycline is increasing, and we recommend that it be used in a combination regimen.

Item Type: Article
Keywords: Antibiotic Susceptibilities Brucella Tigecycline Uniplex PCR
Divisions:
Page Range: pp. 238-247
Journal or Publication Title: Iran J Pathol
Journal Index: Pubmed
Volume: 11
Number: 3
ISSN: 1735-5303 (Print) 1735-5303
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/1612

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