Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Colorimetric immunosensor for determination of prostate specific antigen using surface plasmon resonance band of colloidal triangular shape gold nanoparticles

(2019) Colorimetric immunosensor for determination of prostate specific antigen using surface plasmon resonance band of colloidal triangular shape gold nanoparticles. Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy. p. 8. ISSN 1386-1425

[img] Text
Colorimetric immunosensor for determination of prostate specific antigen using surface plasmon resonance band of colloidal triangular shape gold nanoparticles.pdf

Download (2MB)

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

In this work, we demonstrated the development of a colorimetric immunosensor using surface plasmon resonance band of gold nanoparticles for the detection of prostate specific antigen (PSA). To develop this biosensing tool, triangular gold nanoparticles (AuNPs) were synthesized using Tween-20 as a nonionic surfactant and then, conjugated with PSA capture antibody (Ab(1)-AuNPs). When exposed to Ab(1)-AuNPs, PSA antigens were found to be successfully captured by nanosystem (PSA)-Ab(1)-AuNPs. Next, (PSA)-Ab(1)-AuNPs were incubated with second PSA antibody (2)-decorated magnetite (Fe3O4-Ab(2)) and separated by an external magnetic force to leave Ab(1)-AuNPs in the supernatant solution to be directly analyzed using UV-Vis spectroscopy. It was found that the absorption intensity was directly proportional to the PSA concentration. As a result, the linear range for PSA detection was found to be 0.01-20 ng mL(-1) with a detection limit of 0.009 ng mL(-1). Because of significant stability of the prepared Ab(1)-AuNPs and excellent selectivity to the PSA antigen, this simple and sensitive sensing system is proposed to be potentially effective in the fast and real-time analysis of clinical samples from prostate cancer patients. We believe that the simple platform of this immunosensor to be useful in the development of future point-of-care sensing tools, working on the quantification of biomarkers in a drop of blood. (C) 2019 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Colorimetric immunosensor Sandwich assay Surface plasmon resonance Total prostate specific antigen Magnetic nanoparticles sensitive detection electrochemical immunoassay spectroscopy cancer psa electrochemiluminescence biosensor assay nanocomposite reduction Spectroscopy
Divisions:
Page Range: p. 8
Journal or Publication Title: Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy
Journal Index: ISI
Volume: 222
Identification Number: https://doi.org/10.1016/j.saa.2019.117218
ISSN: 1386-1425
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2301

Actions (login required)

View Item View Item