Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Fabrication of a novel aptasensor based on three-dimensional reduced graphene oxide/polyaniline/gold nanoparticle composite as a novel platform for high sensitive and specific cocaine detection

(2017) Fabrication of a novel aptasensor based on three-dimensional reduced graphene oxide/polyaniline/gold nanoparticle composite as a novel platform for high sensitive and specific cocaine detection. Analytica Chimica Acta. pp. 10-19. ISSN 0003-2670

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Abstract

In the present research, we have developed a novel label free aptasensor based on screen printed carbon electrode (SPCE) modified with three-dimensional magnetic reduced graphene oxide(3D-MRGO)/polyaniline(PA)/gold nanoparticle(AuNP) nanocomposite for impedimetric determination of cocaine. To achieve this goal, a specific thiolated cocaine aptamer was immobilized onto the surface of synthesized nanocomposite. The signaling mechanism of the proposed aptasensor was based on increase in the Fe(CN) 6 (3-/4-) charge transfer resistance (R-CT) as an electrochemical probe in the presence of target analyte. In order to collect of 3D-MRGO/PA/AuNP/aptamer on the surface of working electrode easily, a new electrochemical cell was fabricated. The advantages of the new electrochemical cell configuration can be counted as reusing SPCE for several times, obtaining repeatable responses, reducing required volume of electrolyte and probe solution and making proposed method more user-friendly. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) were used for the characterization of synthesized nanocomposite and modified electrode surface. Under optimized condition, cocaine was determined in a linear concentration range from 0.09 to 85 nM with a detection limit of 0.029 nM by EIS. Also, in order to test applicability of the proposed aptasensor, it was applied to determine cocaine in urine and serum samples and satisfactory results were obtained. (C) 2017 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Electrochemical aptasensor Cocaine Gold nanoparticles Reduced graphene oxide Polyaniline Screen printed carbon electrode chromatography-mass spectrometry solid-phase microextraction label-free gold nanoparticles gas chromatography biological samples carbon electrodes oxide metabolites nanocomposite Chemistry
Divisions:
Page Range: pp. 10-19
Journal or Publication Title: Analytica Chimica Acta
Journal Index: ISI
Volume: 996
Identification Number: https://doi.org/10.1016/j.aca.2017.10.035
ISSN: 0003-2670
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4074

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