Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Anti-cancer effects of Staphylococcal Enterotoxin type B on U266 cells co-cultured with Mesenchymal Stem Cells

(2017) Anti-cancer effects of Staphylococcal Enterotoxin type B on U266 cells co-cultured with Mesenchymal Stem Cells. Microbial Pathogenesis. pp. 438-444. ISSN 0882-4010

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Abstract

Background: Malignant plasma cells are responsible for Multiple Myeloma (MM). Myeloma Cells (MCs) are located in Bone Marrow (BM) and are in contact with stromal cells. The BM-derived Mesenchymal Stem Cells (BMMSCs) affect MCs biology through different mechanisms Currently, Staphylococcal Enterotoxin B (SEB) has been introduced as an anti-tumor agent that is able to kill cancer cells. The present study examined the effects of SEB on MCs and MSCs as an anti-tumor substance. Methods: U266 cellsco-cultured on BM-MSCs and treated with SEB and cell viability was analyzed by MTT assay and flow cytometry. The expression levels of IKKb, IL-6, IL-10, and TGF-beta genes were evaluated by Real Time-PCR technique in U266 cells and BM-MSCs. Results: Data showed that in the presence of SEB, BM-MSCs support U266 cells proliferation and survival. Moreover, SEB, BM-MSCs and BM-MSCs Conditioned Medium (CM) up-regulated IL-6 and IL-10 expression in U266 cells. Additionally, U266 cells showed increased levels in IKKb expression in presence of SEB or BM-MSCs, while expression of IKKb in U266 cells was down -regulated in coexistence of SEB with BM-MSCs or SEB with CM. Also, TGF-beta remained without any changes. Discussion: All in all, SEB can be an appropriate candidate to decrease proliferation and survival rate of cancer cells and it can make noticeable alteration in expression of some genes in U266 cells and BM-MSCs. Further molecular studies are needed to identify the mechanism of action of SEB on U266 cells and BM-MSCs.

Item Type: Article
Keywords: Staphylococcal Enterotoxin B IKKb IL-10 IL-6 TGF-beta Mesenchymal Stem Cell Multiple Myeloma multiple-myeloma cells nf-kappa-b marrow stromal cells growth-factor-beta differentiation factor in-vivo interleukin-10 proliferation induction abnormalities Immunology Microbiology
Divisions:
Page Range: pp. 438-444
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 113
Identification Number: https://doi.org/10.1016/j.micpath.2017.11.024
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4114

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