Abstract

Introduction: Following the activity of the stresssystem, reactions which are generally called fear occur in a person. This fear can causeside effects in individuals such as anxiety and depression. Also one of the important mechanisms of action of stress is production of free radicals and oxidative stress in body. The aim of this study is to evaluate antioxidant system activity and hormonal changes in young male volunteers after experiencing fear and after using Migun bed. Methods: Participants had experienced negative fear and used the Migun bed for 35 minutes. Before and after experiencing fear and using the bed, saliva samples were collected and serum samples were discreted from clot blood. Then alpha-amylase enzyme level and cortisol hormone were assayed by ELISA kit; moreover, superoxide dismutase (SOD) and catalase (CAT) activities, and the levels of glutathione (GSH) and malondialdehyde (MDA) were measured using biochemical methods. Result: alpha-amylase enzyme level after experiencing fear increased and after using the bed also decreased in comparison with pre-fear and pretreatment state. The cortisol level in saliva of individuals after experiencing fear didn't increase and after using the bed decreased significantly in comparison with pre-fear and pretreatment state. Fear significantly increased SOD and CAT activities and MDA level, while GSH level decreased in comparison with pre fear in individuals. Conclusion: Migun Thermal Bed System may also improve the functioning of chief components of the stress axis, and experiencing fear and chronic stress by individuals probably leads to free radical production.