Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair

(2016) Expression of neutrophil gelatinase-associated lipocalin (NGAL) in peripheral nerve repair. Journal of Receptors and Signal Transduction. pp. 429-434. ISSN 1079-9893

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Abstract

Introduction: Trauma is one of the causes of peripheral nerve injuries. Free radicals increase after tissue damage. Free radicals are usually scavenged and detoxified by antioxidants. In this study, we assessed the antioxidative role of the NGAL molecule in sciatic nerve repair in rats. Materials and methods: The sciatic nerves of 40 rats were crushed and the total mRNA of samples from day 1 and 3 and week 1, 3, 5 post injury was extracted. The expression of the NGAL gene was confirmed by RT-PCR. For immunohistochemistry analysis, the samples were fixed in paraformaldehyde and cut in 20 micrometer slices by cryostat. Results: The expression of NGAL significantly upregulated in day 1, 3 and week 1 following the crushing of sciatic nerves in comparison with the intact nerves. Immunohistochemistry results also confirmed the protein expression of this gene. Discussion: The NGAL molecule showed upregulation in the degeneration process after nerve injury, so it may play an important role in nerve repair.

Item Type: Article
Keywords: NGAL peripheral nerve repair sciatic-nerve crush injury oxidative stress rat antioxidant gene regeneration trapidil pattern protein Biochemistry & Molecular Biology Cell Biology
Divisions:
Page Range: pp. 429-434
Journal or Publication Title: Journal of Receptors and Signal Transduction
Journal Index: ISI
Volume: 36
Number: 4
Identification Number: https://doi.org/10.3109/10799893.2015.1132238
ISSN: 1079-9893
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5211

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