Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Screening and identification of SUMP-proteins in sub-acute treatment with diazinon

(2015) Screening and identification of SUMP-proteins in sub-acute treatment with diazinon. Iranian Journal of Basic Medical Sciences. pp. 1240-1244. ISSN 2008-3866

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Abstract

Objective(s): Small ubiquitin-like modifiers (SUMO s) are a family of ubiquitin-related, proteins that are involved in a wide variety of signaling pathways. SUMOylation, as a vital post translational modification, regulate protein function in manycellular processes. Diazinon (DZN), an organophosphate insecticide, causses oxidative stress and subsequently programmed cell death in different tissues. The aim of this study was to evaluate the role and pattern of SUMO modificationas a defense mechanism against stress oxidative, in the heart tissuesof the DZN treated rats. Materials and Methods: Diazinon (15 mg/kg/day), corn oil (control) were administered via gavageto male Wistar rats for four weeks. SUMO1 antibody was covalently crosslinked to protein A/G agarose. heart tissue lysate were added to agarosebeads,After isolation of target proteins (SUMO1- protein)SDS-PAGE gel electrophoresis was performed. Protein bands were identified using MALDI-TOF/TOF and MASCOT). Fold change of (DZN/Ctrl) separated proteins was evaluated using UVband software (UVITEC, UK). Results: Our result showed that subacute exposure to DZN increased SUMOylationoffour key proteins involved in the metabolic process including; Acyl-CoA dehydrogenase, creatine kinase, glyceraldehyde-3-phosphate dehydrogenase and ATP synthase, in the heart tissue of animals.A probability value of less than 0.05 was considered significant (P<0.05). Conclusion: It seems that protein SUMOylation provides a safeguard mechanism against DZN Toxicity.

Item Type: Article
Keywords: Diazinon MALDI-TOF/TOF SUMOlation SUMO1 creatine-kinase oxidative stress protective role rats sumoylation pathway crocin cells Research & Experimental Medicine Pharmacology & Pharmacy
Divisions:
Page Range: pp. 1240-1244
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: ISI
Volume: 18
Number: 12
ISSN: 2008-3866
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5295

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