Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Design of a chimeric DNA vaccine against Brucella spp

(2014) Design of a chimeric DNA vaccine against Brucella spp. Minerva Biotecnologica. pp. 223-233. ISSN 1120-4826

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Abstract

Brucellosis, a common illness between domestic animals and humans, is mostly caused by Brucella (B) abortus, B. melitensis and B.suis. It has been shown vaccination of mice by Omp19 and Omp31 can induce protection against different Brucella spp. Furthermore, urease enzyme is considered a virulence factor in most members of Brucella. Here, we designed a chimeric DNA vaccine containing omp19, omp31 and urease genes to obtain high protection against Brucella spp. The construct was analyzed using bioinformatics tools. Linear and discontinuous B-cell epitopes, MHC class I and II binding peptides of the chimeric vaccine were predicted. Results suggest the construct can be an appropriate vaccine candidate against brucellosis.

Item Type: Article
Keywords: Brucellosis Vaccines, DNA Zoonoses outer-membrane protein-31 heat-shock proteins t-cell epitopes escherichia-coli subcellular-localization expression vectors recombinant omp31 immune-responses fusion protein b-melitensis Biotechnology & Applied Microbiology
Divisions:
Page Range: pp. 223-233
Journal or Publication Title: Minerva Biotecnologica
Journal Index: ISI
Volume: 26
Number: 4
ISSN: 1120-4826
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5656

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