Abstract

Aim. The aim of this study was to investigate chemical composition (GC/MS analysis), antioxidant (DPPH) and antibacterial (MIC analysis) activities of three extracts obtained from Scrophularia striata. Methods. The extraction of Scrophularia striata plant was extracted through ethanolic, hydro alcoholic and aqueous methods. The chemical composition of the three extracts is determined by GC-mass analysis method. DPPH assay was performed for measurement of antioxidant activity. MIC and MBC were determined with micro well dilution assey. Results. GC-MS analysis revealed that ethanolic and aqueous extracts were rich in Bis(2-ethylhexyl) phthalate (25.17, 27.855 respectively) and hydro alcoholic extract in Oleyl Alcohol (24.81). All of the extracts had the antibacterial activity. Among all extracts, hydro alcoholic extract indicated the most activity against pathogenic bacteria, and had high antioxidant activities as measured by DPPH scavenging (0.407 mg/mL). The aqueous extract had the lowest amount of total phenolic compounds (88.42 +/- 2.55 mg). Conclusion. A strong correlation was observed between the antibacterial, radical scavenging capacity and the phenolic and flavonoid compounds of the extracts. As expected, amounts of the total phenolic and flavonoid components were very high in the hydro alcoholic extract.