Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Diagnosis of streptococcus pneumoniae based on cpsB, cpsD, and psaA genes

(2018) Diagnosis of streptococcus pneumoniae based on cpsB, cpsD, and psaA genes. Journal of Mazandaran University of Medical Sciences. pp. 114-119. ISSN 17359279 (ISSN)

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Abstract

Background and purpose: Fast diagnosis of pneumococcal infections is of great importance in achieving successful outcomes. The aim of this study was to use PCR process based on capsular encoding genes of Streptococcus pneumonia. Materials and methods: In this experimental study, specific primers were designed based on the reference sequences of the Streptococcus pneumoniae cpsB, cpsD, aroE, and psaA genes. All bacteria were subjected to DNA extraction separately. Then, the PCR protocol was performed. Sequencing of the PCR products was done and data was analyzed descriptively. Results: The primer pairs against the cpsB, cpsD, aroE, and psaA genes amplified 159, 202, 254, and 286 bp fragments, respectively. The alignments and comparison of amplified sequences showed a high homology (100) with the reference genes. Conclusion: According to this study, it is possible to diagnose Streptococcus pneumoniae based on virulence genes, and differentiate the pathogenic Streptococcus pneumoniae from nonpathogenic strains and also viridans groups. Therefore, this method is suggested in diagnosis of pathogenic Streptococcus pneumoniae strains. © 2018, Mazandaran University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: Capsular genes PCR Streptococcus pneumoniae aroe gene Article bacterial gene cpsb gene cpsd gene DNA extraction nonhuman polymerase chain reaction psaa gene Streptococcus pneumonia
Divisions:
Page Range: pp. 114-119
Journal or Publication Title: Journal of Mazandaran University of Medical Sciences
Journal Index: Scopus
Volume: 28
Number: 161
ISSN: 17359279 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/637

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