Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Detection of H9N2 avian influenza virus in various organs of experimentally infected chickens

(2011) Detection of H9N2 avian influenza virus in various organs of experimentally infected chickens. African Journal of Microbiology Research. pp. 5826-5830. ISSN 1996-0808

[img] Text
Detection of H9N2 avian influenza virus in various organs of experimentally infected chickens.pdf

Download (163kB)

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

H9N2 Avian influenza virus (AVI) infection is a major cause of economic losses in poultry industry. Therefore further study to explain the virus pathogenesis is necessary. In this study tissue tropism and dissemination of A/chicken/Iran/11T/99(H9N2) virus in various organs of specific pathogen free (SPF) chickens were investigated. Fifty 2-week-old chickens hatched from SPF eggs were divided randomly into two groups. Forty chicks in the experimental and ten chicks in the control group. Experimental chicks were inoculated intranasally-intraorally with the virus. Samples of lung, trachea, pancreas, thymus, spleen, brain, bursa of fabricius, proventriclus, cloaca and kidney were aseptically collected at 1, 3, 5, 7, 9 and 10 day post inoculation (DPI). A reverse transcriptase polymerase chain Reaction (RTPCR) test was performed for virus detection. Viral RNA was detected in the respiratory system on days 3, 5 and 7 PI. The virus was also found in the kidney on days 3,5,7,9 PI and in the pancreas on days 3 and 5 PI. Viral RNA was observed only on day 5 PI in cloaca. The virus was not detected in the blood, brain and immune system. The virus was not found from any organs on day 10 PI. These results suggest that H9N2 AIV has tropism for respiratory, digestive and urinary system following intranasal/intraoral inoculation.

Item Type: Article
Keywords: Avian influenza H9N2 SPF RT-PCR a virus comparative pathology duck-origin pathogenesis h7 Microbiology
Divisions:
Page Range: pp. 5826-5830
Journal or Publication Title: African Journal of Microbiology Research
Journal Index: ISI
Volume: 5
Number: 32
Identification Number: https://doi.org/10.5897/ajmr11.214
ISSN: 1996-0808
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6443

Actions (login required)

View Item View Item