Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Immunogenicity of a plant-derived edible chimeric EspA, Intimin and Tir of Escherichia coli O157:H7 in mice

(2011) Immunogenicity of a plant-derived edible chimeric EspA, Intimin and Tir of Escherichia coli O157:H7 in mice. Plant Science. pp. 620-627. ISSN 0168-9452

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Abstract

Transgenic plants offer the possibility to produce and deliver an oral immunogen on a large-scale with low production costs and minimal purification or enrichment. Cattles are important reservoirs of Escherichia coli O157:H7 and developing a specific immunity in animals would be invaluable. Intimin, Tir, and EspA proteins are the virulence factors expressed by LEE locus of enterohemorrhagic E. coli. We hypothesized that the chimeric recombinant forms of these effectors delivered as an edible-base vaccine would reduce colonization of bacteria in mice. A synthetic gene (eit) composed of espA (e), eae (i) and tir (t) attached by linkers was constructed. The gene was codon optimized and cloned into plant expression vectors adjacent to CaMV35S and FAE promoters for expression in tobacco and canola plants. Of total soluble protein 0.2 and 0.3 (in average) were detected in transgenic tobacco leaves and canola seeds respectively. Mice immunized either subcutaneously or orally with recombinant EIT and challenged with E. coli 0157:H7 significantly exhibited reduced bacterial shedding. Application of transgenic plants containing trivalent immunogen is an effective tool for protection against E. coli O157:H7. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Keywords: Escherichia coli O157:H7 Transgenic plants Type III secreted proteins Vaccine iii-secreted proteins brassica-napus l. b surface-antigen lt-b intestinal colonization o157-h7 infection oral immunization subunit vaccine gene transformation Biochemistry & Molecular Biology Plant Sciences
Divisions:
Page Range: pp. 620-627
Journal or Publication Title: Plant Science
Journal Index: ISI
Volume: 180
Number: 4
Identification Number: https://doi.org/10.1016/j.plantsci.2011.01.004
ISSN: 0168-9452
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6571

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